Equipment and services

See also...

Wide-field systems (fluorescence and brightfield-equipped):

Zeiss Axioimager Z1
Zeiss Axiovert 200M
Zeiss P.A.L.M. Microbeam III

Laser-scanning systems:

Zeiss LSM510 confocal
Zeiss LSM510 META NLO multiphoton

Zeiss Axioimager Z1
Zeiss Axioimager Z1 upright motorized microscope (with 5x, 10x, 20x, 40x and 63x Plan APO objectives and TRITC, GFP and DAPI filter sets) equipped with both an Axiocam HrC (color) camera and a nuance multispectral imaging system for spectral scanning and unmixing.

Fee structure
Assisted rate: $35/hr.
Unassisted rate: $13/hr.

Zeiss Axiovert 200M
Zeiss Axiovert 200M inverted microscope with Axiocam MrC camera (with 2.5x Plan Neofluar, 10x Plan APO, 20x LD-A Plan, 20x Plan Apo and 40x Plan Apo objectives and filter sets for DAPi, rhodamine and FITC).

The Imaging Core currently subsidizes fees for the Axiovert 200.

Zeiss P.A.L.M. Microbeam III
Zeiss P.A.L.M. Microbeam III laser capture microdissection system (with 10x Fluar, 20x Neofluar, 40x Plan Neofluar and 63x Plan Neofluar objectives, with DAPI, TRITC and FITC filters).

Fee structure
Assisted rate: $60/hr.
Unassisted rate: $25/hr.
Training (per person): $100/hr.

Zeiss LSM510
Zeiss LSM510 laser scanning confocal inverted microscope equipped with 405 diode (for DAPI), multiline argon (458nm, 477nm, 488nm, 514nm), DPSS561 (568nm), red HeNe (633nm) lasers, and EC Plan Neofluar 5x and 10x, Plan Apo 20x, LD C-Apochromat 40x, and C-Apo 63x objectives.

Fee structure
Assisted rate: $80/hr.
Unassisted rate: $50/hr.
After hour user unassisted rate: $25/hr.
Training (1 session = 9 hrs./person): $360/session

Zeiss LSM510 META
Zeiss LSM510 META NLO multiphoton laser scanning microscope equipped with an Argon multiline laser (458nm, 477 nm, 488 nm, 514 nm), a DPSS561 (568nm) laser, a red HeNe laser (633 nm), and a Coherent Chameleon Ultra II Ti: sapphire laser, rapidly tunable between 680 and 1080 nm. This system is outfitted with a Zeiss XL-3 incubator to assure stable environmental control during long incubations, and with Zeiss EC Plan Neofluar 5x and 10x, Plan Apo 20x, C-Apo 40x, and W Plan-Apo objectives.

Software systems include Axiovision 4.6 and the basic LSM software, supplemented by modules for multichannel fluorescence, extended focus, interactive measurement, deconvolution, multitime and rapid 3D reconstruction.

Fee structure
Assisted rate: $100/hr.
Unassisted rate: $55/hr.
After hour user unassisted rate: $25/hr.
Training (1 session = 11 hrs./person): $500/session

	Zeiss Axioimager Z1 Zeiss Axioimager Z1 upright motorized microscope (with 5x, 10x, 20x, 40x and 63x Plan APO objectives and TRITC, GFP and DAPI filter sets) equipped with both an Axiocam HrC (color) camera and a nuance multispectral imaging system for spectral scanning and unmixing. Fee structure Assisted rate: $35/hr. Unassisted rate: $13/hr.



	*Zeiss Axiovert 200M* Zeiss Axiovert 200M inverted microscope with Axiocam MrC camera (with 2.5x Plan Neofluar, 10x Plan APO, 20x LD-A Plan, 20x Plan Apo and 40x Plan Apo objectives and filter sets for DAPi, rhodamine and FITC). The Imaging Core currently subsidizes fees for the Axiovert 200.



	Zeiss P.A.L.M. Microbeam III Zeiss P.A.L.M. Microbeam III laser capture microdissection system (with 10x Fluar, 20x Neofluar, 40x Plan Neofluar and 63x Plan Neofluar objectives, with DAPI, TRITC and FITC filters). Fee structure Assisted rate: $60/hr. Unassisted rate: $25/hr. Training (per person): $100/hr.



	Zeiss LSM510 Zeiss LSM510 laser scanning confocal inverted microscope equipped with 405 diode (for DAPI), multiline argon (458nm, 477nm, 488nm, 514nm), DPSS561 (568nm), red HeNe (633nm) lasers, and EC Plan Neofluar 5x and 10x, Plan Apo 20x, LD C-Apochromat 40x, and C-Apo 63x objectives. Fee structure Assisted rate: $80/hr. Unassisted rate: $50/hr. After hour user unassisted rate: $25/hr. Training (1 session = 9 hrs./person): $360/session



	Zeiss LSM510 META Zeiss LSM510 META NLO multiphoton laser scanning microscope equipped with an Argon multiline laser (458nm, 477 nm, 488 nm, 514 nm), a DPSS561 (568nm) laser, a red HeNe laser (633 nm), and a Coherent Chameleon Ultra II Ti: sapphire laser, rapidly tunable between 680 and 1080 nm. This system is outfitted with a Zeiss XL-3 incubator to assure stable environmental control during long incubations, and with Zeiss EC Plan Neofluar 5x and 10x, Plan Apo 20x, C-Apo 40x, and W Plan-Apo objectives. Software systems include Axiovision 4.6 and the basic LSM software, supplemented by modules for multichannel fluorescence, extended focus, interactive measurement, deconvolution, multitime and rapid 3D reconstruction. Fee structure Assisted rate: $100/hr. Unassisted rate: $55/hr. After hour user unassisted rate: $25/hr. Training (1 session = 11 hrs./person): $500/session