Developmental Vascular Biology Program
Current events/important calendars
Ramani Ramchandran, PhD, professor
The Developmental Vascular Biology Program, under the directorship of Dr. Ramchandran, is home to graduate students, post-doctoral fellows, undergraduate students and faculty. Dr. Ramchandran began his research career at the Georgia Health Sciences University (formerly called Medical College of Georgia) in Dr. Dorothy Tuan's laboratory in 1992 where he studied the transcriptional mechanisms controlling hematopoiesis. He then joined Dr. Vikas Sukhatme's laboratory in 1997 as a post-doctoral fellow at Beth Israel Deaconess Medical Center, Harvard Medical School. In Dr. Sukhatme's laboratory he studied the role of basement membrane proteins in endothelial cell growth and the implications of this process for tumor growth. His work at Harvard primarily focused on understanding the mechanisms of angiogenesis, the growth of new blood vessels from pre-existing vessels, and how this process affects tumor growth. In 2002, he was recruited to the NIH on receipt of the National Cancer Institute Scholar Award, and established his first independent research program studying the developmental mechanisms of vascular biology. In 2007, he was recruited to the Children's Research Institute at the Medical College of Wisconsin where he currently serves on the faculty as a professor in the Department of Pediatrics. At CRI, Dr. Ramchandran made a serendipitous discovery that led to the identification of mutations in two genes sucrose non-fermenting related kinase-1 (snrk-1) and dual specific phosphatase-5 (dusp-5) in patients with vascular anomalies. Since then his research has focused on the role of these genes in both normal and abnormal vascular development process in disease. He has also established a robust drug discovery program that is geared towards identifying small molecules targeting critical gene products responsible for diseases affected by deregulated vascular growth. firstname.lastname@example.org (414) 955-2387
Kathy Thorpe, administrative assistant
I support the Developmental Vascular Biology Program in terms of its day-to-day academic activities, and manage most of the logistics associated with these activities for Dr. Ramchandran, post doctoral fellows and technicians in his lab. Some of my administrative duties include coordinating grant submissions in eBridge, travel arrangements and reimbursement, CVs, budgets, scheduling meetings and human resource matters. In addition, I serve as an Evacuation Marshall, and am in the process of earning my CERT and CRP email@example.com (414) 955-2946
8701 Watertown Plank Road
Milwaukee, WI 53226
Michelle Bordas, research technologist
I am interested in all aspects of animal care and research. My role in the lab is to maintain our mice colony. I maintain the colony in accordance with the guidelines and regulations for animal care use in research. Through my effort and those of other lab members, we are able to complete many valuable research projects while upholding the standards of best possible animal care. Together we are committed to the humane and appropriate use of animals for research. firstname.lastname@example.org (414) 955-2453
Jaladhi Nayak, research associate
My research involves the study of lymphatic malformations such as generalized lymphatic anomaly (GLA) and Gorham-Stout syndrome (GSS), which later lead to abnormal bone resorption. Currently, I am focusing on characterizing the effects of cell-cell communication between lymphatic endothelial cells and osteoclasts and its potential role in GLA and GSS pathogenesis. I am also currently working on protein expression and purification of a crucial protein involved in vascular development. email@example.com (414) 955-2274
Stephanie Cossette, PhD, post-doctoral fellow
My research focuses understanding the role of sucrose non-fermenting related kinase 1 (SNRK1) during embryonic development. SNRK1 is involved in the metabolism-sensing pathway and is regulated by liver kinase B1 (LKB1), which is a tumor suppressor that is also associated with the Peutz-Jeghers syndrome. By using the mammalian system to systematically remove SNRK1 from various types of tissue/organ populations, I will be able to investigate the specific role of SRNK1 in development as well as investigate the developmental relationship between SNRK1 and LKB1. firstname.lastname@example.org (414) 955-2393
Adam Gastonguay, PhD, post-doctoral fellow
Phosphatases play a critical role in many signaling pathways by tightly regulating the duration of kinase driven cell signaling. In addition to normal cell signaling, phosphatases have also been shown to regulate cell development, differentiation, and cancer. I'm interested in the discovery and characterization of novel small molecule inhibitors against phosphatases involved in cancer and vascular malformations. Not only will this research yield compounds that are invaluable tools for phosphatase driven research, but it may also yield compounds with possible therapeutic potential. email@example.com (414) 955-2950
Chris Koceja, technician
My research entails combining two different transgenic approaches to over-express oncogenes in sympathetic neurons of the zebrafish nervous system, while utilizing the GAL4/UAS system. firstname.lastname@example.org (414) 456-5896
Noah Leigh, MS, research associate
My research interests are focused on drug discovery related to the process of angiogenesis. Currently I am working on the characterization of a novel gene DU644441 that I worked on for my Masters thesis. My goal is to determine the cell type the gene is expressed in during embryonic development in zebrafish and to confirm this gene is a matrix metalloproteinase (MMP) by conducting MMP assays using expressed protein. email@example.com (414) 955-2392
Keguo Li, PhD, research scientist
My research interest lies in understanding the significance of intergenic transcripts. Specifically, I am interested in the roles of the transcripts in the flanking regions of Dll4 locus. We use quantitative visualizing technology with single molecular sensitivity to track such transcripts during vascular development. Gain- and loss-of-function studies are utilized to probe the impact of the transcripts on angiogenesis. Another intriguing field to me is the positional cloning of subtelomeric genes. firstname.lastname@example.org (414) 456-7356
Adam Sabel, zebrafish lab technician
I am responsible for managing two of our zebrafish facilities in the MFRC and CRI research buildings. My research interest is in maintaining small animal health and more specifically in terms of fish – maintaining overall fish health, breeding, and nursery. I also keep logs of our fish stocks and help maintain consistent water parameters (pH, conductivity, temp.) in our fish rooms. We have now transitioned to an electronic fish database for record keeping. I intend to further expand my research interests in other aspects of fish developmental biology. email@example.com (414) 955-4878
Marcus Schupp, PhD, post-doctoral fellow
My work aims to unravel molecular mechanisms controling the patterning and cellular behavior of vascular progenitor cell populations during early steps of vertebrate vasculogenesis. In particular, by using zebrafish transgenesis and imaging technology, I will investigate the gene-regulatory networks underlying the specification and differentiation of vasculogenic precursors and investigate signaling pathways affecting their proliferative and migratory properties. firstname.lastname@example.org (414) 955-2557
Padmanabhan Vakeel, PhD, post-doctoral fellow
Kinases and phosphatases play a major role in all cellular functions of the cell and have wider implications in the disease state. My investigations are focused on understanding their intricate connection during vasculogenesis and angiogenesis using model systems such as zebrafish, mouse, and mammalian cell culture. The GOF/LOF (Gain/Loss of function) studies on these molecules in model systems would reveal the spatial and temporal nature of their function within the complex cellular signaling network. email@example.com (414) 955-2963
Ling Wang, PhD, post-doctoral fellow
My research interest is to understand the pathogenesis of cardiovascular disease and cancer, and applying this knowledge to treatment in the clinic. Currently, I am focused on elucidating the fundamental vasculogenesis mechanisms using a variety of cell culture, zebrafish and mouse models, and to explore the contribution of the vasculogenic process to tumor growth. firstname.lastname@example.org (414) 955-2390
Sreenivas Kilari, PhD, post-doctoral fellow
At present, I have two research interests based on my current projects. In the first project, I am focusing on the VEGFR-2/KDR signaling as modified by ECSCR (Endothelial Cell Specific Chemotaxis Receptor) during vascular development. In a second project, I am examining ephrin-B2 signaling partners in endothelial cells. email@example.com (414) 955-2273
Raman Kutty, graduate student
I work on refining and adding to our understanding of vascular growth and development. Specifically, my graduate work focuses achieving better understandings of the etiological mechanisms of vascular tumors such as hemangiomas. Currently, I am developing in vitro techniques and animal models to better understand the role of dual specific phosphatase-5 (dusp-5), particularly its role in endothelial cell signaling. Concurrently, I am using these advancements to aid in the development of small molecule targets of dusp-5, for use in the treatment of hemangiomas. As an MD/PhD candidate, translational efforts such as these are at the heart of my medical education and will ultimately serve as the cornerstone for my professional career. firstname.lastname@example.org (414) 955-5470
Research in the Developmental Vascular Biology Program
The Developmental Vascular Biology program investigates the basic mechanisms of blood vessel formation in vertebrates and the contribution of the vasculature to disease states. In particular, vascular conditions associated with children such as hemangiomas and solid tumors will benefit because ongoing studies in this program utilize developing zebrafish and mouse embryo model systems.
We study how a specified endothelial precursor cell (angioblast) eventually differentiates into artery or vein. The basic mechanisms of this process often are dysregulated in disease. Therefore, understanding the basic mechanisms of vessel formation will generate new treatments for conditions affected by deregulated vessel growth. In particular, vascular conditions associated with children such as hemangiomas and solid tumors will benefit because ongoing studies in this program utilize developing zebrafish and mouse embryos. Zebrafish studies specifically are primarily embryonic in nature and thus directly contribute to child development. In addition, tools for performing drug screens using zebrafish embryos are being developed, which will identify targets and potential drug leads for treating pediatric vascular conditions.
During development blood vessels adapt to specific needs of the organ they supply. One highly vascularized organ system is the lungs. In the lungs, the vasculature becomes highly specialized to provide efficient gas-exchange by participating with alveoli. Development of lung alveoli requires complex interactions among blood vessels, other specialized cells and the extracellular matrix. Defects in any one of these elements will adversely affect alveolar development. Human lung pathologies including emphysema and infant bronchopulmonary dysplasia are associated with defective alveolar structure and concomitant vascular pathologies.
Other research projects
- Angioblast development in vertebrates
Endothelial precursor cells or angioblasts are specified from lateral mesoderm cells in the developing vertebrate embryo. The intermediate steps necessary for the angioblast to form arteries and veins are not clear. The Ramchandran laboratory studies the signals and processes involved in the different steps of angioblast development in a developing embryo using a variety of cell biology, genetic and molecular approaches.
- Role of axon guidance genes in vascular development
Vessels and nerves are branching networks that often lay side by side in a developing embryo. Recently, mechanisms governing branching morphogenesis are shared both at the cell surface and the intracellular levels in endothelial cells and axons respectively. The Ramchandran lab is interested in understanding the molecular mechanisms that guide an endothelial cell to its target using clues from the axon guidance system. We study one member of the axon guidance family, roundabout4 (robo4), and its role in this process
- Translational disease models and Drug Discovery
Vessel growth is tightly regulated during development. Any imbalance in this regulation often is associated with disease. For example, tumor growth is dependent on neo-vessel growth or angiogenesis. The Ramchandran laboratory is developing disease models in fish using a variety of genetic and molecular approaches that will eventually be used for drug screening. We have recently launched computational small molecule and conventional screening approaches to target mutant protein vs. wild type protein in the vasculature. Using such approaches we have identified candidate small molecules against targets mutated in human vascular anomaly disease. The efficacy of some compounds is in the nanomolar range, which is being further developed as therapeutics. In addition, the Ramchandran lab has taken a leading role in forming a drug discovery consortium in Southeastern Wisconsin with the focus on merging individual expertise across participating institution laboratories in SE Wisconsin towards a common goal of generating affordable therapeutics that will benefit the health of the local communities and beyond.
Important research technique/methodology papers
- The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments Stephen A. Bustin,1* Vladimir Benes,2 Jeremy A. Garson,3,4 Jan Hellemans,5 Jim Huggett,6 Mikael Kubista,7,8 Reinhold Mueller,9 Tania Nolan,10 Michael W. Pfaffl,11 Gregory L. Shipley,12 Jo Vandesompele,5 and Carl T. Wittwer13,14
- Generation of Transgenic Zebrafish Using Tol2 Thomas S. Becker Lab, Pavla Navratilova
- ZF Sectioning Nature Protocols Jessica Sullivan-Brown, Margaret E. Bisher & Rebecca D. Burdine
Resources for gene-specific reagents
Public aggregator sites
The NCBI Gene Page provides annotation including PubMed links and (at the bottom) aggregates cDNA, small nucleotide and antiserum links.
Bioinformatics Harvester gathers data from a number of gene pages, protein predictions and also links to products related to specific genes. Entry is via text search and is not very good with alternate gene names.
- Biocompare lists antisera, short nucleotide reagents and small molecules from about 20 vendors. Searchable by text, does not typically recognize alternate names.
- Exact Antigen (To be renamed Labome in 2010) lists antisera, short nucleotide reagents and cDNA clones from multiple vendors. Text search is very robust and will identify alternative names for gene products.
- Origene sells expression constructs including C-terminal fusion constructs for many human and mouse gene products. Also sells short nucleotide reagents.
- Open Biosystems has a very large set of cDNAs and nucleotide reagents. Searchable by text or BLAST.
- Invitrogen sells a large number of gene-specific reagents.
- Genecopoeia sells a wide variety of gene-specific reagents, including Gateway-ready entry constructs.
Mutant allele collections
- ITGC, the International Gene Trap Consortium, lists a large number of mouse ES cell lines with gene trap integration intended to generate null alleles.
- The Sanger Institute Zebrafish Mutation Resource generates zebrafish mutant lines by ENU mutagenesis followed by exon sequencing recovery. Users can request a gene to be targeted with delivery time of up to 12 months.
- http://www.fishnet.org.au/ This site has optical position tomography images of zebrafish sections. Use this site to refer to anatomical location of the structure that you are interested in identifying.
- http://www.anaspec.com/products/productcategory.asp?id+857 This company sells a few antisera against zebrafish antigens. They also sell de-yolked lysates, probably by a similar protocol to ours. The name of the company is Eurogentec Anaspec.
- 2002-2008 – National Institutes of Health NCI Scholar Award, Ramani Ramchandran, PhD
- 2004 - Fellow Award for Research Excellence (FARE), Outstanding Intramural Research, Dayadevi Jirage, PhD
- 2007-2010 – Advancing a Healthier Wisconsin Cloche: A basic and translational model for caridiovascular disease
- 2007-2008 - State of Wisconsin Breast Cancer Tax Check-Off Program, Post-doctoral award, Title: Intracellular Signaling Mechanisms of Robo-4, a Putative Anti-Angiogenic Target, Ganesh Samant, PhD
- 2008-2009 - Estate of Debra Ann Bosque and Richard Wiedehold & Elizabeth Brinn Foundation, Post-doctoral award, Title: Intracellular Signaling Mechanisms of Robo-4, a Putative Anti-Angiogenic Target, Ganesh Samant, PhD
- 2008 - Angioblast development in zebrafish development, Young Investigator Award, 3rd Mayo Clinic Angiogenesis Symposia, Changzoon Chun, PhD
- 2008 - Travel Awards – Ramchandran Lab Received for 3rd Mayo Angiogenesis Symposia
- 2008 - EphrinB2 role in mouse lung vascular development, Young Investigator Award, 3rd Mayo Clinic Angiogenesis Symposia, George Wilkinson, PhD
- 2009 - Identification of the physiologically relevant ligand for the angiogenic Roundabout-4 (Robo4) receptor, American Heart Association Mid-West Affiliate Post Doctoral Fellowship, Ganesh Samant, PhD
- 2010 - Natural antisense transcript-mediated gene regulation in zebrafish vasculature, American Heart Association Mid-West Affiliate Post Doctoral Fellowship, Keguo Li, PhD
- 2010 - Activation of VEGF receptors during zebrafish angioblast migration, CRI Pilot Innovative Research Grant. Medical College of Wisconsin, George Wilkinson, PhD
- 2010 - Travel Award from MCW Graduate School for Zebrafish, Conference in Madison, Wisconsin, Noah Leigh
- 2010 - ECSCR, a novel enhancer of VEGF-dependent migration, American Heart Association Scientist Development Grant Award, George Wilkinson, PhD
- 2011 - Research Training Fellowship funded through a T35 Training Grant from National Heart, Lung, Blood Institute, National Institutes of Health, Scott Brunson
- 2011 - A novel approach to the identification of candidate genes in patients with lymphatic malformations. Medical College of Wisconsin Research Day 2011, Poster, Kelly Duffy, PhD, Christopher Johnson, M.D., Ganesh Samant PhD, Stephanie Cossette, Jennifer Santoro and Ramani Ramchandran, PhD
- 2012 - Research Training Fellowship funded through a T35 Training Grant from National Heart, Lung, Blood Institute, National Institutes of Health, Raman Kutty
- 2012 - Research Training Fellowship funded through a T35 Training Grant from National Heart, Lung, Blood Institute, National Institutes of Health, Colin Stair
- 2012 - Established Investigator Award, American Heart Association. Grant was awarded but programmatic eligibility criteria not met, Ramani Ramchandran, PhD
- 2012 – Travel Awards from Mayo Clinic for 5th Mayo Clinic Angiogenesis Symposium, Minneapolis, Minnesota, Stephanie Cossette, PhD, Padmanabhan Vakeel, PhD, Ling Wang, PhD, Sreenivas Kilari, PhD
- 2013 - Distinguished Alumnus Award, Medical College of Georgia
Invited presentations (2010-present)
- Developmental vasculogenesis mechanisms during embryogenesis and disease in vertebrates. Beihang University of Aeronautics and Astronautics, Beijing, China
- Enigma of long-non-coding RNA in Vertebrate Biology. The Chinese University of Hong Kong, Hong Kong, China
- Developmental vasculogenesis mechanisms during embryogenesis and disease in vertebrates.Department of Biology and Center for Chinese Medicine, Hong Kong University of Science and Technology Clear Water Bay Road. Hong Kong, China
- Tools for a Successful Career in Science. K.J.Somaiya College of Science and Commerce, Somaiya Vidyavihar Complex, Vidyavihar-East, Mumbai, India
- Vasculogenesis mechanisms during vertebrate embryonic development.AU-KBC Research Centre, Anna University, Chennai, India
- A Robo:Sox Nexus in Angiogenesis, International Conference of Angiogenesis: Basics and Applications, Chennai, India
- The role of matrix in tumor metastasis, CSIR-Indian Institute of Chemical Technology. Hyderabad, India
- Transcriptional Regulation During Cardiac Progenitor Development, The 7th Oriental Congress of Cardiology. Shanghai, China
- Vascular Development in Vertebrates. Physiology and Biophysics, University of Louisville School of Medicine, Louisville, KY
- Vasculogenesis mechanisms in a developing vertebrate. Cardiovascular Seminar Series, University of Virginia Health System, Charlottesville, VA
- Dusp-5: a putative target for vascular disease.Caliper Life Sciences, San Francisco, CA
- Vasculogenesis mechanisms in a developing vertebrate. Mayo Angiogenesis Symposia, Mackinac Island, MI
- Dusp-5: A valued target identified by serendipity.Institute for Therapeutics Discovery & Development, University of Minnesota, Minneapolis, MN
- A Robo:Sox nexus in vascular development.Children's Environmental Health Sciences Core Center, External Advisory Committee Meeting, Intercontinental Hotel, Milwaukee, WI
- Bridging the gap from targets to drugs (Valley of Death): A proposal for a SE WI Drug Development Consortium. Concordia University of Wisconsin, Mequon, WI
- Signaling molecules in vascular development and disease.Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN
- Transcriptional mechanisms regulating embryonic angiogenesis. Department of Biochemistry & Molecular Biology at Wayne State University, School of Medicine - Detroit, MI
- Serendipitous discovery implicates signaling molecules in vascular Anomalies. Physiology Department, Georgia Health Sciences University, Augusta, GA
- Transcriptional Regulation of Roundabout4 (Robo4) in the Developing Vasculature. Biochemistry Department, Georgia Health Sciences University, Augusta, GA
- Novel targets for the treatment of pediatric vascular tumors and anomalies. Emory University School of Medicine, Atlanta, GA
- The role of sox and robos in angiogenesis. 5th Mayo Clinic Angiogenesis Symposium, Minneapolis, MN
- Transcriptional regulation of etv-2 during cardiovascular development in vertebrates.University of Kansas Medical Center, Kansas City, KS
- Vascular anomalies: Insights from developmental biology. Louisiana State University, Shreveport, LA
- The Vascular Channel That Guided My Career – From Bombay to GRU and beyond! Georgia Regents University, Augusta, GA
- The Role of Astrocytes in Tumor Cell Invasion and Metastasis. Georgia Regents University, Augusta, GA
- Role of lymphatic endothelial cells and osteoclasts in lymphatic anomalies. First International Conference on Generalized Lymphatic Anomalies and Gorham's Disease, Washington, DC
- ETV2 transcriptional regulation during cardiac progenitor cell Development. University of Oklahoma Health Science Center, Oklahoma City, OK
- Functional characterization of Snrk-1 gene in developing vasculature - an update. CVRC Works-in-Progress
- Vasculogenesis mechanisms in vertebrates. CBNA Presentation
- How to make sense of anti-sense RNA? CRI Noon Conference
- Early mechanistic steps of vasculogenesis process revealed in zebrafish.June Multidisciplinary Research Conference
- Successful Strategies for Science and Life.Summer Research Lecture Series
- Small Molecule Identification for a Vascular Target: Dusp-5. Pediatric Surgery Multidisciplinary Research Conference
- Bridging the gap from targets to drugs (Valley of Death): A proposal for a SE WI Drug Development Core
- Targeting Dusp-5: An emerging option for treating vascular anomalies.CTSI Seminar Presentation
- Insights on Aortic Coarctation Defects from Studying Embryonic Vasculogenesis Process in Zebrafish.Children's Research Institute Scientific Symposium
- Axon guidance genes and their role in developing vasculature. Cardiovascular Center
- Identification of small molecules for treatment of vascular tumors. Cancer Cell Biology Research Forum
- Insights from zebrafish studies on clinical conditions associated with congenital heart defects.HHC Grand Rounds
- Basic mechanisms and treatment strategies for pediatric tumors: A zebrafish model system perspective.Pediatric Hematology/ Oncology/ BMT Faculty Meeting
- Molecular Targets for the Treatment of Cardiac and Vascular Anomalies.CRI Research Conference
- Vascular Development Using Zebrafish Models.Friday Morning Lecture Series
- Developmental Vascular Biology Program. Interdisciplinary Program Lunch Series Faculty Talks
- Transcriptional regulation in cardiac progenitor cells during embryonic development. Cardiovascular Research Focus Meeting, Herma Heart Center, Medical College of Wisconsin, Milwaukee, WI
- Garnaas MK, Liu M, Marx R, Li K, Baraban J, Horowitz A, Ramchandran R. Syx, a novel Rho A guanine exchange factor, is essential for angiogenesis in vivo 2008. Circulation Research 2008 Sep 26;103(7):710-6; PMID: 18757825.
- Chun CZ, Kaur S, Samant GV, Wang L, Pramanik K, Garnaas M, Li K, Field L, Mukhopadhyay D, and Ramchandran R. Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates. Blood 2009, 113(5):1192-1199; PMID: 18723694. **Inside Blood commentary on both articles.
- Pramanik K, Chun CZ, Garnaas MK, Samant GV, Li K, Horswill MA, North PE, and Ramchandran R. Dusp-5 and Snrk-1 coordinately function during vascular development and disease. Blood 2009, 113(5):1184-1191; PMID: 18927432. **Inside Blood commentary on both articles.
- Kaur S*, Samant GV*, Pramanik K, Loscombe PW, Pendrak ML, Roberts DD, and Ramchandran R. Silencing of directional migration in robo4 knockdown endothelial cells. BMC Cell Biology 2008 Nov 39:61; PMID: 18980679.
- Makky K, Duvnjak P, Pramanik K, Ramchandran R and Mayer AN. A whole-animal microplate assay for metabolic rate using zebrafish. Journal of Bio Molecular Screening 2008 Dec; 13(10):960-7; PMID: 19029015.
- Jia Y, Wu S-L, Isenberg JS, Sipes JM, Field L, Zeng B, Bandle RW, Ramchandran R, Karger BL, and Roberts DD. Thiolutin inhibits endothelial cell adhesion by perturbing Hsp27 interactions with the actin cytoskeleton. Cell Stress Chaperones 2010 Mar; 15(20:165-81; PMID: 19579057.
- Kanungo J, Zhen, Y, Amin N, Kaur S, Ramchandran R, and Pant, H. Specific inhibition of cyclin-dependent kinase 5 activity induces motor neuron development in vivo 2009. Biochemical and Biophysical Research Communications 386 (2009):263-267; PMID: 19523926.
- Li K, Blum Y, Verma A, Liu Z, Pramanik K, Leigh N, Chun C, Samant G, Zhao B, Garnaas M, Horswill M, Stanhope S, North P, Miao R, Wilkinson G, Affolter M, and Ramchandran R. A noncoding antisense RNA at the tie-1 locus regulates tie-1 transcript levels function in vivo. Blood 2010 Jan7;115(1):133-9; PMID: 19880500.
- Verma A, Bhattacharya R, Remadevi I, Li K, Pramanik K, Samant G, Horswill M, Chun C, Mukhopadhyay D, Ramchandran R, and Wilkinson G. Ecscr promotes angioblast migration during vasculogenesis and enhances kdr sensitivity. Blood, 2010 Jun 3; 115(22):4614-22; PMID: 20086248. **Inside Blood commentary on EC-specific chemotaxis receptor: a double-edged sword, p.4614.
- Zhao B, Chun C, Liu Z, Horswill M, Pramanik K, Wilkinson G, Ramchandran R, and Miao R. Nogo-B receptor is essential for angiogenesis in vivo via Akt pathway. Blood, 2010. Dec 9;116(24):5423-33. PMID: 20813898.
- Chun CZ, Remadevi I, Schupp M, Samant GV, Pramanik K, Wilkinson GA and Ramchandran R. Fli+ etsrp+ hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafish. PLoS One. 2011 Feb 25;6(2):e14732; PMC3045372.
- Lakshmikanthan S, Sobczak M, Chun CZ, Henschel A, Dargatz J, Ramchandran R, Chrzanowska-Wodnicka M. Rap1b regulates angiogenesis by an endothelial cell-autonomous mechanism involving integrin ávâ3-dependent VEGFR2 activation. Blood 2011. In press. PMID not available.
- Samant GV, Schupp MO, François M, Moleri S, Kothinti RK, Chun CZ, Sinha I, Sellars S, Leigh N, Pramanik K, Horswill MA, Remadevi I, Li K, Wilkinson GA, Tabatabai NM, Beltrame M, Koopman P, and Ramchandran R. Sox factors transcriptionally regulate robo4 expression in the developing vasculature in zebrafish. J. Biol. Chemistry 2011. PMID: 21730073; PMC3162435.
- Patra C, Kim J, Pramanik K, d'Uscio LV, Patra S, Ramchandran R, Strano M, and Mukhopadhyay D. Reactive Oxygen Species Driven Angiogenesis by Inorganic Nanorods. PMID 21967244.
- 1997 - Interview and picture appeared in the MCG Today magazine regarding graduate students' perspective on current issues such as NIH funding.
- October 15, 1999 - Organization brings together Indian scientists. Bulletin in Focus (Harvard Medical School publication)
- Summer 1999 - Intent on killing cancer - Scientists move closer to a new approach. Basic Research in Pulse (Beth israel Deaconess Medical Center)
- May 4, 2001 - First cell surface receptor identified for endostatin. Research brief in Focus (Harvard Medical School publication)
- August 2006 - PNAS paper highlighted in the CCR newsletter, Frontiers in Science http://ccr.cancer.gov/news/frontiers/august2006/ramchandran.asp
- March 2007 – Our research program has been highlighted in the "Searchlight" e-newsletter (Children's Research Institute, Medical College of Wisconsin)
- March 2007 – Faculty appointment – Press release – Medical College of Wisconsin
- May 2007 – Investigation of Blood Vessel Formation Strives to Understand Cancer Tumor Growth – Article in MCW Cancer Center News (Medical College of Wisconsin)
- October 2007 - State Tax Check-Off Program Raises $115,000 for MCW Breast Cancer Research – Ganesh Samant, post-doctoral fellow award announcement
- 2008 - Kaur S*, Samant GV*, Pramanik K, Loscombe PW, Pendrak ML, Roberts DD and Ramchandran R. Silencing of directional migration in robo4 knockdown endothelial cells 2008. BMC Cell Biology in press. BMC Cell Biology Article figure chosen as "image of month" for publication online. http://www.biomedcentral.com/bmccellbiol/imageofthemonth/2008/12 BMC Cell Biology Article: Highly Accessed Article Citation given based on number of downloads in a given month for this article
- January 29, 2009 – Press coverage concerning article - Dusp-5 and Snrk-1 coordinately function during vascular development and disease 2008. Blood 2009, 113 (5):1184-1191. Birthmark or Blood Vessel Problem?
- The Washington Post http://www.washingtonpost.com/wp-dyn/content/article/2009/01/29/AR2009012903270.
- News Wise http://www.newswise.com/articles/view/548143/.
- MSN Health and Fitness http://health.msn.com/kids-health/articlepage.aspx?cp-documentid=100232148.
- Forbes.com http://www.forbes.com/feeds/hscout/2009/01/29/hscout623304.
- Yahoo News http://news.yahoo.com/s/hsn/20090130/hl_hsn/birthmarkorbloodvesselproblem.
- Womenshealth.gov (The federal government source for women's health) http://www.womenshealth.gov/news/english/623304.
- The Investigator (Children's Research Institute newsletter) http://chw.org/vascularbiology
- April 2009, Medical College of Wisconsin Receives Grant to Study Vascular Tumor Proteins, MCW Press Release http://infoscope.mcw.edu/NewsCenter/CollegeNews/Collegereceivesgranttostudyvasculartumorproteins.htm
1R01HL090712-01A2 (Ramchandran, R.) 2009-2014 - NIH/NHLBI. Roundabout4 signaling in endothelial cells – Role: PI
1R01HL102745-01 (Ramchandran, R.) 2011-2015 - NIH/NHLBI. Snrk-1 and Dusp-5 co-ordinately regulate vascular development in vertebrates - Role: PI
1R01HL112639-01 (Ramchandran, R.) 2011-2016 - NIH/NHLBI. Targeting DUSP-5 to Treat Vascular Anomalies - Role: PI
1R01HL108938-01 (Miao, R. and Ramchandran, R.) 2011-2016 NIH Role of NgBR - Role: Co-I
1R01HL111582-01 (Wodnicka, M. and Ramchandran, R.) 2012-2017 - NIH. Rap1 in VEGF signaling in endothelial cells - Role: Co-I
GRANT10842376 (Ferrante, A and Ramchandran, R) 2012-2014 - NIH. Fully automated angiogenesis drug discovery assay in zebrafish - Phase II - Role: Co-I
NA (Gershan, J. and Ramchandran, R.) 2013-2014 - Basic Sciences Research Program Seed Grant, Medical College of Wisconsin Cancer Center (MCWCC). Identification of Factors that Promote Invasion and Metastasis of Epithelial Tumor Cells - Role: Co-I
HL120585 (Mukherjee, P. and Ramchandran, R.) 2013-2018 - NIH. Cystathionine beta synthase (CBS) and angiogenesis - Role: Co-I
HL007792 (Harder, D) 2009-2014 - NIH T32 Training Program. Hypertension and Vascular Biology Training Grant - Role: Mentor